The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Exploring RNA transcription and turnover in vivo by using click chemistry. Capturing the interactome of newly transcribed RNA. Transcriptome-wide discovery of coding and noncoding RNA-binding proteins. ![]() Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila. The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs. The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. iCLIP: protein-RNA interactions at nucleotide resolution. PAR-CliP-a method to identify transcriptome-wide the binding sites of RNA binding proteins. The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3. DNMT1-interacting RNAs block gene-specific DNA methylation. A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Local regulation of gene expression by lncRNA promoters, transcription and splicing. Gene silencing by microRNAs: contributions of translational repression and mRNA decay. How cells get the message: dynamic assembly and function of mRNA-protein complexes. RNA binding protein regulation and cross-talk in the control of AU-rich mRNA fate. OOPS is compatible with downstream proteomics and RNA sequencing, and can be applied in any organism. We also characterize dynamic changes in RNA–protein interactions in mammalian cells following nocodazole arrest, and present a bacterial RNA-interactome for Escherichia coli. OOPS is approximately 100-fold more efficient than existing methods and can enable analyses of dynamic RNA–protein interactions. We show that all long RNAs can be cross-linked to proteins, and recovered 1,838 RBPs, including 926 putative novel RBPs. We validated OOPS in HEK293, U2OS and MCF10A human cell lines, and show that 96% of proteins recovered were bound to RNA. We present orthogonal organic phase separation (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it to recover cross-linked protein–RNA and free protein, or protein-bound RNA and free RNA, in an unbiased way. Apply conditioner.Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated RNAs, including long noncoding RNA, pre-mRNAs and bacterial RNAs. DO NOT repeat more than 2 to 3 times (and always depending on condition of hair and scalp). If condition of hair permits, repeat above steps. If additional color removal is needed, be certain to check hair condition, texture and porosity. (The more you rinse the better your results.) Check for amount of color removal. Shampoo again, and rinse for another 5 minutes ? towel blot hair. ![]() (Additional rinsing is needed for longer, thicker hair.) This step is very important for desired results. Rinse with warm water and shampoo, then rinse for 15-20 minutes. While processing, be certain that the room is NOT cold and be certain that you are not sitting near a vent blowing cool air, as this will affect timing and results. Gently re-work through for even penetration. Be certain there is even and adequate product penetration throughout the areas of hair that color is being removed. Move quickly through the application as the activity of the mixture decreases with elapsed time. Apply liberally to areas of hair that need hair color removal or correction. While pointing bottle away from face, remove cap and place applicator nozzle on applicator bottle 2 (cut nozzle tip if necessary). Replace cap on applicator bottle 2 and shake well for 30 seconds. ![]() Pour entire contents of bottle 1 into applicator bottle 2. While pointing bottle away from face, remove cap on applicator bottle number 2.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |