The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Exploring RNA transcription and turnover in vivo by using click chemistry. Capturing the interactome of newly transcribed RNA. Transcriptome-wide discovery of coding and noncoding RNA-binding proteins. ![]() Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila. The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs. The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. iCLIP: protein-RNA interactions at nucleotide resolution. PAR-CliP-a method to identify transcriptome-wide the binding sites of RNA binding proteins. The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3. DNMT1-interacting RNAs block gene-specific DNA methylation. A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Local regulation of gene expression by lncRNA promoters, transcription and splicing. Gene silencing by microRNAs: contributions of translational repression and mRNA decay. How cells get the message: dynamic assembly and function of mRNA-protein complexes. RNA binding protein regulation and cross-talk in the control of AU-rich mRNA fate. OOPS is compatible with downstream proteomics and RNA sequencing, and can be applied in any organism. We also characterize dynamic changes in RNA–protein interactions in mammalian cells following nocodazole arrest, and present a bacterial RNA-interactome for Escherichia coli. OOPS is approximately 100-fold more efficient than existing methods and can enable analyses of dynamic RNA–protein interactions. We show that all long RNAs can be cross-linked to proteins, and recovered 1,838 RBPs, including 926 putative novel RBPs. We validated OOPS in HEK293, U2OS and MCF10A human cell lines, and show that 96% of proteins recovered were bound to RNA. We present orthogonal organic phase separation (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it to recover cross-linked protein–RNA and free protein, or protein-bound RNA and free RNA, in an unbiased way. Apply conditioner.Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated RNAs, including long noncoding RNA, pre-mRNAs and bacterial RNAs. DO NOT repeat more than 2 to 3 times (and always depending on condition of hair and scalp). If condition of hair permits, repeat above steps. If additional color removal is needed, be certain to check hair condition, texture and porosity. (The more you rinse the better your results.) Check for amount of color removal. Shampoo again, and rinse for another 5 minutes ? towel blot hair. ![]() (Additional rinsing is needed for longer, thicker hair.) This step is very important for desired results. Rinse with warm water and shampoo, then rinse for 15-20 minutes. While processing, be certain that the room is NOT cold and be certain that you are not sitting near a vent blowing cool air, as this will affect timing and results. Gently re-work through for even penetration. Be certain there is even and adequate product penetration throughout the areas of hair that color is being removed. Move quickly through the application as the activity of the mixture decreases with elapsed time. Apply liberally to areas of hair that need hair color removal or correction. While pointing bottle away from face, remove cap and place applicator nozzle on applicator bottle 2 (cut nozzle tip if necessary). Replace cap on applicator bottle 2 and shake well for 30 seconds. ![]() Pour entire contents of bottle 1 into applicator bottle 2. While pointing bottle away from face, remove cap on applicator bottle number 2.
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![]() It has a free version but the full version of CamScanner brings higher quality scans, no ads or watermark and ability to edit OCR results. It allows you to scan notes, forms, documents, receipts, and images and convert. With the Adobe Scan app, easily capture and convert documents, forms, business cards, and whiteboards into high-quality PDFs. Kaagaz Scanner Scanner App Made in India. Home Best Products Productivity Capture Important Data With the Best Scanning and OCR Apps Turn tax paperwork into PDFs, make a digital copy of your titles and deeds, and save other important. Adobe Scan is one of the best scanner apps for Android. There’s also a cool Search feature, that lets you search for invoices and receipts from the past.ĬamScanner also supports various cloud storage services like Dropbox, Box, Google Drive, Evernote and OneDrive. Here is a list of the best Indian scanner apps in 2023 as CamScanner alternatives: DocScanner. Since the app is available on all major platforms including iOS and Windows Phone, there’s ability to sync documents on different platforms. It brings the ability to enhance scans, annotate them, extract texts from images and collaborate with others. ![]() CamScanner lets you scan documents, receipts, business cards, notes on whiteboard, contracts or paperwork, certificates, ID cards and more. There are no surprises here, as CamScanner is undoubtedly the best scanner app on Android and other platforms as well. Here are the Best Scanner Apps for Android: CamScanner There are a ton of scanner apps on Google Play Store and we tried a lot of them and found the best ones for you. We were sceptical at first but after using some scanner apps on Android, we are pleased to say, they work great and the quality of scans is certainly good enough. It works similarly to detect phone numbers, email addresses, and URLs. If you have been wondering whether these scanner apps can match the quality of a real scanner, they cannot but they have gotten very close indeed. Smart Lens is a highly recommended Adobe Scan alternative for Android. ![]() ![]() So, not only do you get the satisfaction of laying the smackdown on your enemies, but you also get to walk away with some snazzy prizes. While some things simply heal your Pokémon, others can be game-changers that significantly enhance your battle prowess. These can range from health-boosting elixirs to weapons that help you dish out more damage. Speaking of rewards, the game showers you with many items post-battle. After the battle, you’ll score various items, some for healing, some for attacking-think of it as a post-fight swag bag. Yes, you heard it right-Mega Evolve! That’s like turning your already-awesome dragon into an even more incredible, I-don’t-even-know-what-that-is-but-it’s-cool dragon. Here’s the kicker: Pokémon retain their original powers and even can Mega Evolve in this game. What’s a Pokémon game without some good old-fashioned fisticuffs, right? The battles here are turn-based, offering various actions from attacks to defensive maneuvers. So, before you venture too deep into a dungeon, ensure you’re prepared to dodge attacks, evade traps, and hopefully make it out with some valuable loot. Think of it as Black Friday shopping but for magical items and with the risk of, you know, actual mortal danger. It’s like LinkedIn for Pokémon-networking is critical! DUNGEON EXPLORATION: WHERE TREASURE MEETS TROUBLEĭungeons in this game aren’t just dark, spooky caverns they’re crawling with treasures and, unfortunately, a healthy dose of enemies and traps. Along the way, you can recruit other Pokémon to help you on your quest. In essence, your job is to discover why you’ve undergone this drastic change and, if possible, how to return to your human self. In this version, you’re more of a persuasive diplomat than a Pokémon poacher. Instead, you’ll have to win their hearts and minds by completing specific tasks for them. Now, this isn’t your traditional Pokémon game where capturing and defeating Pokémon is the name of the game. It’s a high school with scales, fur, and elemental powers. Here, you’ll meet friends who can make your school experience either a dream or a nightmare. Not only do you need to adapt to your new shape-shifting life, but you also have to attend a school designed exclusively for Pokémon youngsters. Each comes with its own set of strengths and attributes. In Pokémon Super Mystery Dungeon, you’ll wake up to find yourself mysteriously transformed into one of 20 selectable Pokémon. TRANSFORMATION AND SCHOOLING – A JOURNEY BEGINSĮver thought high school was hard? Try doing it as a Pokémon. Welcome to the captivating world of Pokémon Super Mystery Dungeon on the Nintendo 3DS. Now, you find yourself enrolled in a school for young Pokémon, making friends, dodging bullies, and navigating the complexities of being a pocket monster. ![]() Before you can say, “What the Bulbasaur?” a Nuzleaf gallantly swoops in to fend off the bad vibes, giving you time to hightail it. The next, you’re a full-blown Pokémon, rudely greeted by Beheeyem’s aggressive antics. Imagine this: one moment, you’re just an average Joe (or Jane). ![]() ![]() In another scene, we simply get to enjoy her wear a bikini that shows off her delicious curves and swerves. Look at her shapely thighs and booty as she lowers her body into the tub. ![]() Katherine Moennig (Shane) Has Sex With A Bridesmaid Part 1 0:20. Edita Brychta in nude scene from Akumulator 1. The clip has been taken from the movie named CXibalba / Curse of the Mayans. Is it really there.or is there something far more sinister that lays in the caves? Of course, it's the second thing! While ancient terror lurks beneath the water looking to punish humanity for disturbing it, we are here to get hot and horny with Carla Ortiz. Carla Ortiz shows off her exquisite hourglass figure when she takes it all off and dips her body into the bathtub. Carla Ortiz just sexy, she shows her naked ass and boobs there. Watch Carla Ortizs Sexy scene on AZNude for free (7 minutes and 13 seconds). What are they looking for? The lost library of the Mayans is supposed to contain thousands of codexes and other texts that give all of the answers of the Mayans. Watch Carla Ortizs Sexy scene on AZNude for free (7 minutes and 13 seconds). They go into the jungles south of the Yucatan and dive into the underwater caves of Xibalba. The archeologist believes that this will be the biggest discovery of all time and it turns out that he was right. Expert diver Danielle Noble and her crew of divers are taken with the archeologist for the journey and she has a genuine interest in ancient history and legends. Carla Chambel puts on a tank top in front of a mirror, scene in 98 Octanas. Carla Chambel breasts, sexy scene in 98 Octanas 0:17. Raquel Welch strips off for X-RATED sex scene in very steamy throwback from The Beloved RAQUEL WELCH became a huge pin-up back in the 1960’s, and looking at this sex scene from The Beloved, it. Carla Chambel Nude bathes in the lake, scene in 98 Octanas 1:51. Curse of the Mayans (2017) is a horror and sci-fi film from director Joaquin Rodriguez about an American archeologist who leads a team of cave divers to search for ancient Mayan artifacts and records that might be buried in deep sea caves. NUDESHARK.COM: carla ortiz videos » Simple. To manually keep track of all of your receipts require far more effort. From those vendors, WellyBox automatically collects the receipt from the portal itself, all you need to do is click on the Sync button on the left-hand side of the WellyBox dashboard, find the company portal you want and click on “Connect”. įor Your Information – Some companies have closed portals and they don’t send you a receipt on the email.
![]() Special thanks to Jessica Meyer, Lanisha Newstead, Becca Rees, Ashley Wilkinson and Maayan Dar for your constant support, helping us to make the most out of this amazing tool. □Ī huge thank you as well to our amazing Product, Analytics and Marketing teams at Wallapop for all the work, enabling us to release proudly soon to our community our 2022 Salseo. Huge thanks to Braze for the opportunity of attending and sharing during those 2 intense days. We had the amazing opportunity to present one of our latest project: the Wallanniversary, our very first annual Wrapped Campaign, aka Salseo, at Wallapop - who aims at celebrating loyalty and building community through strengthening the emotional connection with our brand. □ This is, of course, only if the extreme gravity doesn’t destroy the observer first.Last week, María Alba Mata and I attended the annual Braze Forge Conference in London. ![]() For example, there is a point in the axis of symmetry that has the property that if an observer is below this point, the pull from the singularity will force the observer to pass through the middle of the ring singularity to the region with closed time-like curves and it will experience repulsive gravity that will push it back to the original region, but then it will experience the pull from the singularity again and will repeat this process forever. There are some other interesting free-fall trajectories. This interior solution is not likely to be physical and is considered a purely mathematical artefact. Since the trajectory of observers and particles in general relativity are described by time-like curves, it is possible for observers in this region to return to their past. The region beyond permits closed time-like curves. The spacetime allows a geodesic curve (describing the movement of observers and photons in spacetime) to pass through the center of this ring singularity. This kind of singularity has the following peculiar property. Since a point cannot support rotation or angular momentum in classical physics (general relativity being a classical theory), the minimal shape of the singularity that can support these properties is instead a ring with zero thickness but non-zero radius, and this is referred to as a ringularity or Kerr singularity. With a fluid rotating body, its distribution of mass is not spherical (it shows an equatorial bulge), and it has angular momentum. This is not the case with a rotating black hole (a Kerr black hole). When a spherical non-rotating body of a critical radius collapses under its own gravitation under general relativity, theory suggests it will collapse to a single point. PEP 655 - Marking individual TypedDict items as required or potentially-missingĪnd now for something completely different.PEP 675 - Arbitrary Literal String Type.On average, we measured a 1.22x speedup on the standard benchmark suite. Python 3.11 is up to 10-60% faster than Python 3.10. The Faster CPython Project is already yielding some exciting results.gh-34627 - Atomic grouping ( (?>.)) and possessive quantifiers ( *+, ++, ?+, +) are now supported in regular expressions.gh-90908 - Introduce task groups to asyncio. ![]() PEP 680 - tomllib: Support for Parsing TOML in the Standard Library.PEP 654 - Exception Groups and except*.PEP 657 - Include Fine-Grained Error Locations in Tracebacks.Some of the new major new features and changes in Python 3.11 are: General changes Major new features of the 3.11 series, compared to 3.10 Python 3.11.0 is the newest major release of the Python programming language, and it contains many new features and optimizations. ![]() This is the stable release of Python 3.11.0 Convenience: Purchasing a fake marriage certificate online is a hassle-free option. ![]() There are several advantages to buying a fake marriage certificate online:ġ. Benefits of buying a fake marriage certificate online It is always recommended to purchase fake marriage certificates from trusted online providers to ensure compliance with local laws and regulations. While it may be tempting to create a fake marriage certificate on your own, it is crucial to consider the legal implications and potential consequences of using a fraudulent document. Personal reasons: Some individuals may require a fake marriage certificate for personal reasons, such as celebrating an anniversary or as a novelty item.ĭocument replacement: If you have lost or misplaced your original marriage certificate, a fake certificate can serve as a replacement for decorative or nostalgic purposes. Movie or theater productions: Fake marriage certificates are commonly used as props in films, TV shows, or theater productions. Pranks or jokes: Fool your friends and family with a fake marriage certificate for laughter-inducing pranks. This type of certificate is often used for various purposes, such as: Ensure that the certificate offered meets your specific requirements and looks authentic.Ī fake marriage certificate is a replica of a genuine marriage certificate, designed to appear as authentic as possible. When looking to buy a marriage certificate online, it is crucial to find a reputable and reliable provider. Whether you require a marriage certificate for legal purposes, personal reasons, pranks, or as a prop for a movie or theater production, purchasing a fake marriage certificate online can provide a convenient solution. Are you in need of a marriage certificate but don’t want to go through the hassle of organizing a wedding and dealing with legal procedures? Look no further! With the advancement of technology, it is now possible to buy a marriage certificate online. ![]() If you are interested in using an image for a publication, please visit for more information and to fill out the online Image Rights and Reproductions Request Form. Located in the heart of Silver Lake, California, Pine & Crane, a fast-casual Taiwanese restaurant, delivers authentic, delicious Asian fare in a modern setting.Named after chef and owner Vivian Ku's grandfather's noodle company, the restaurant's philosophy revolves around memories and family - all of the freshly harvested produce comes from her family's nearby Asian vegetable farm. A pine tree arches across the background above. A crane stands in the foreground, with it's head and beak turned toward the viewer, revealing a patch of orangish red on its face. This painting inculdes the seal of the artist Ren Xun. ![]() To suggest the cold season, Ren Xun chose orange pigment to depict the pine needles. This elegant painting of a crane, a symbol of immortality, and pine and bamboo, symbols of longevity, is appropriate for birthday or New Year’s gifts. Ren Xun, best known for painting birds and animals, was the younger brother of Ren Xiong’s (1823–1857). Pine and bamboo are symbols of longevity and the crane is a symbol of immortality, making this painting appropriate for a birthday or New Year’s gift. To suggest the cold season, Ren Xun uses orange pigment to depict the needles of the pine tree positioned between the bamboo and the crane. His dramatic brushwork may be seen in the calligraphic lines of the bamboo in the background, which contrast boldly with the controlled ink washes of the crane’s plumage. Ren Xun was best known for painting birds and animals. ![]() He remained active until around 1887, when his eyesight began deteriorating. When Ren Xun was twenty-two years old his brother died and he continued to build his artistic career, eventually becoming famous throughout the Yangzi River region. Ren Xiong’s son Ren Yu, his distant nephew Ren Yi (whose work is also on display), and the two brothers are known as the “Four Rens,” who dominated late nineteenth-century painting in Shanghai. Ren Xun studied painting with his elder brother Ren Xiong (1823–1857), whose work is also on view in this gallery. Museum Purchase made possible by the Friends of the Museum of Art “We were actually up there just trying to get ready for the season,” said Thompson, the principal investigator of the species since 2010. ![]() This year, researchers spotted their first Mount Charleston blue on June 17, the earliest sighting on record. The adult butterflies only live a week or two, generally taking flight between late June and the end of August to mate and lay eggs during the short window of warm weather in the high country. The exact population is unknown, but there have been years when surveyors couldn’t find any of the insects. For example, Thompson said, no one has ever seen the insect in its larval, caterpillar stage, which is thought to last up to two years. There is a lot researchers still don’t know about the species. The males sport iridescent blue-and-gray wings to attract mates, while the dull, bluish-brown females flutter about depositing tiny eggs on one of two small, ground-hugging host plants. The Mount Charleston blue is a distinctive subspecies of the wider-ranging Shasta blue butterfly. In 2015, federal regulators designated more than 5,200 acres in the Spring Mountains, including portions of the Lee Canyon ski resort, as critical habitat for the insect. The Mountain Charleston blue butterfly was added to the list of endangered species in September 2013, one month after the Carpenter 1 fire was contained at almost 28,000 acres. “The first plants that have come back in these burned areas between the trees are the host plants of the butterfly and the nectar plants of the butterfly,” he said. Based on the plants his team has seen growing this summer and last, Thompson said it looks like the Carpenter 1 Fire in 2013 may have opened up a sizable new addition to the butterfly’s habitat. “The more places they are located across the mountain top, the less likely that one disturbance could take the entire population out,” Thompson said.Īnd that’s not the only good news he had to offer. Forest Service also found a few Mount Charleston blues on a second ski run at Lee Canyon, suggesting the butterfly might be expanding its range there. This year, the team of researchers and students from UNLV, the U.S. UNLV biologist and butterfly expert Daniel Thompson said it’s been 20 years since anyone has counted as many butterflies along the Bonanza Trail as his group saw in 20. ![]() The Mount Charleston blue butterfly is still incredibly rare, but researchers are seeing more of them in more places than they have in decades.Ĭlose to 200 of the endangered insects were spotted over the summer at several locations high in the Spring Mountains, including isolated patches of previously unknown habitat from Bonanza Peak to the ridge line above the Lee Canyon ski area. A male Mount Charleston blue butterfly collects nectar from a rock goldenrod flower in this undated photo taken in the Spring Mountains. Always ask what deals or discounts are available. Here are a few major gyms that offer trial periods: Gym Many gyms offer free passes for first-timers to their business. The option to try a gym membership before you buy it can be a great way to see if it’s a good fit for you. ![]() To find your nearest community center, you can search the web for recreation centers in your city or county. You might not find specialized workout equipment at your community center gym, but you’re likely to get a great deal on access to standard gym equipment and may also have group class options. Where I live, the community center gym is free for city residents, but non-resident adults can pay a $3 daily fee or just $15 a month. The best deals on community center gyms usually go to city or county residents, but many centers offer low rates to non-residents. For anyone age 55 or older, the cost drops to $65 a year, or less than $6 a month. So, for about $9 per month, residents can access the weight rooms, walking tracks, swimming pools and more in 33 public recreation centers across the city. In the city of Atlanta, for example, a one-year athletic pass for adults costs $110. Community CentersĬommunity centers can be a great way to get access to a gym for practically - if not completely - free. For workouts that only require standard weight training or cardio equipment (like dumbbells, treadmills, ellipticals, stationary bikes, etc.), the following options can save you money on your gym membership. If you don’t need a gym for your workouts, I share alternative fitness options that are free or practically free below.īut if you want a gym membership, the type of gym you go to will impact what you pay. What type(s) of workouts do you want to do?Īccording to Finder, “Americans spend $397 million on unused gym memberships annually.” The simplest way to save money on a gym membership is to not get one that’ll go unused.Ways To Save Money on a Gym Membershipīefore you join a gym, here are two questions to consider: Details of notable changes can be found here. This article was updated in December 2023 and I review it every six months. Other Fitness Options That Are Practically Free.But I’ve looked into how you can get a membership for less. ![]() On average, a gym membership costs around $700 per year. With monthly membership fees, one-time joining fees, taxes and more, the costs to join a gym can add up quickly. |
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